Lu LinHouhui SongQichao TuYujia QinAifen ZhouWenbin LiuZhili HeJizhong ZhouJian Xu2016-01-082016-03-302016-01-082016-03-302011-10-13Lin L, Song H, Tu Q, Qin Y, Zhou A, Liu W, et al. (2011) The Thermoanaerobacter Glycobiome Reveals Mechanisms of Pentose and Hexose Co-Utilization in Bacteria. PLoS Genet 7(10): e1002318. doi:10.1371/journal.pgen.1002318http://hdl.handle.net/11244/23530Author Summary Renewable liquid fuels derived from lignocellulosic biomass could alleviate global energy shortage and climate change. Cellulose and hemicellulose are the main components of lignocellulosic biomass. Therefore, the ability to simultaneously utilize pentose and hexose (i.e., co-utilization) has been a crucial challenge for industrial microbes producing lignocellulosic biofuels. Certain thermoanaerobic bacteria demonstrate this unusual talent, but the genetic foundation and molecular mechanism of this process remain unknown. In this study, we reconstructed the structure and dynamics of the first genome-wide carbon utilization network of thermoanaerobes. This transcriptome-based co-expression network reveals that glucose, xylose, fructose, and cellobiose catabolism are each featured on distinct functional modules. Furthermore, the dynamics of the network suggests a distinct yet collaborative nature between glucose and xylose catabolism. In addition, we experimentally demonstrated that these novel network-derived features can be rationally exploited for product-yield enhancement via optimized timing and balanced loading of the carbon supply in a substrate-specific manner. Thus, the newly discovered modular and precisely regulated network elucidates unique features of thermoanaerobic glycobiomes and reveals novel perturbation strategies and targets for the enhanced thermophilic production of lignocellulosic biofuels.en-USAttribution 3.0 United StatesXylose,Glucose,Thermoanaerobacter,Catabolism,Pentoses,Hexoses,Glucose metabolism,FructosesThe Thermoanaerobacter Glycobiome Reveals Mechanisms of Pentose and Hexose Co-Utilization in BacteriaResearch Article10.1371/journal.pgen.1002318false