Friedline, AnthonyZachariah, MalcolmMiddaugh, AmyHeiser, MattKhanna, NeerajVaishampayan, ParagRice, Charles V.2015-04-202016-03-302015-04-202016-03-302015-04-17Friedline, Anthony, et al. Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide. AMB Express 2015, 5:24http://hdl.handle.net/11244/14437Bacillus pumilus SAFR-032 spores isolated from a clean room environment are known to exhibit enhanced resistance to peroxide, desiccation, UV radiation and chemical disinfection than other spore-forming bacteria. The survival of B. pumilus SAFR-032 spores to standard clean room sterilization practices requires development of more stringent disinfection agents. Here, we report the effects of a stabilized chlorine dioxide-based biocidal agent against spores of B. pumilus SAFR-032 and Bacillus subtilis ATCC 6051. Viability was determined via CFU measurement after exposure. Chlorine dioxide demonstrated efficacy towards sterilization of spores of B. pumilus SAFR-032 equivalent or better than exposure to hydrogen peroxide. These results indicate efficacy of chlorine dioxide delivered through a stabilized chlorine dioxide product as a means of sterilization of peroxide- and UV-resistant spores.Bacillus pumilus ; Bacillus subtilis ; Chlorine dioxide; Spore killing; SporesArticle10.1186/s13568-015-0109-4