2017-02-062017-02-062016-12-15NUCLEAR FACTOR Y, Subunit A (NF-YA) Proteins Positively Regulate Flowering and Act Through FLOWERING LOCUS T Siriwardana CL, Gnesutta N, Kumimoto RW, Jones DS, Myers ZA, et al. (2016) NUCLEAR FACTOR Y, Subunit A (NF-YA) Proteins Positively Regulate Flowering and Act Through FLOWERING LOCUS T. PLOS Genetics 12(12): e1006496. doi: 10.1371/journal.pgen.1006496http://hdl.handle.net/11244/48165Photoperiod dependent flowering is one of several mechanisms used by plants to initiate the developmental transition from vegetative growth to reproductive growth. The NUCLEAR FACTOR Y (NF-Y) transcription factors are heterotrimeric complexes composed of NF-YA and histone-fold domain (HFD) containing NF-YB/NF-YC, that initiate photoperiod-dependent flowering by cooperatively interacting with CONSTANS (CO) to drive the expression of FLOWERING LOCUS T (FT). This involves NF-Y and CO binding at distal CCAAT and proximal “CORE” elements, respectively, in the FT promoter. While this is well established for the HFD subunits, there remains some question over the potential role of NF-YA as either positive or negative regulators of this process. Here we provide strong support, in the form of genetic and biochemical analyses, that NF-YA, in complex with NF-YB/NF-YC proteins, can directly bind the distal CCAAT box in the FT promoter and are positive regulators of flowering in an FT-dependent manner.20 pages3,348,008 bytesapplication.pdfAttribution 3.0 United StatesArabidopsis thaliana -- FloweringPlants, Flowering of -- Molecular aspectsArabidopsis thaliana -- Molecular geneticsPlant genetic regulationArticle10.1371/journal.pgen.1006496