Ryan A. ZanderJenna J. GuthmillerAmy C. GrahamRosemary L. PopeBradly E. BurkeDaniel J.J. CarrNoah S. Butler2017-03-052017-03-052016-10-12Zander RA, Guthmiller JJ, Graham AC, Pope RL, Burke BE, Carr DJ, et al. (2016) Type I Interferons Induce T Regulatory 1 Responses and Restrict Humoral Immunity during Experimental Malaria. PLoS Pathog 12(10): e1005945. doi:10.1371/journal.ppat.1005945http://hdl.handle.net/11244/49301We thank Christopher Hunter and Bob Axtell for critical feedback, and the Flow Cytometry Laboratory at OUHSC for technical assistance.Author Summary Humoral immunity is essential for host resistance to pathogens that trigger highly inflammatory immune responses, including Plasmodium parasites, the causative agents of malaria. Long-lived, secreted antibody responses depend on a specialized subset of CD4 T cells called T follicular helper (Tfh) cells. However, anti-Plasmodium humoral immunity is often short-lived, non-sterilizing, and immunity rapidly wanes, leaving individuals susceptible to repeated bouts of malaria. Here we explored the relationship between inflammatory type I interferons, the regulation of pathogen-specific CD4 T cell responses, and humoral immunity using models of experimental malaria and systemic virus infection. We identified that type I interferons promote the formation and accumulation of pathogen-specific CD4 T regulatory 1 cells that co-express interferon-gamma and interleukin-10. Moreover, we show that the combined activity of interferon-gamma and interleukin-10 limits the magnitude of infection-induced Tfh responses, the secretion of parasite-specific secreted antibody, and parasite control. Our study provides new insight into the regulation of T regulatory 1 responses and humoral immunity during inflammatory immune reactions against systemic infections.en-USAttribution 3.0 United StatesT helper cells,Parasitic diseases,Plasmodium,Humoral immunity,Malaria,Malarial parasites,Cloning,AntibodiesResearch Article10.1371/journal.ppat.1005945false